Coding

Part:BBa_K4733004:Design

Designed by: Diego Perez Hidalgo   Group: iGEM23_UBC-Okanagan   (2023-10-05)


Hyperfolder Yellow Fluorescent Protein (hfYFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The UBC-Okanagan 2023 team used Modular Cloning (MoClo) standardization for this part: added Type II restriction sites and standard flanks to this part.



Source

This part was engineered by Campbell et al. (2022). The sequence was made available in the Protein Data Bank (PDB) by the investigators.

References

Campbell, B. C., Paez-Segala, M. G., Looger, L. L., Petsko, G. A., & Liu, C. F. (2022). Chemically stable fluorescent proteins for advanced microscopy. Nature Methods, 19(12), 1612–1621. https://doi.org/10.1038/s41592-022-01660-7